Detailansicht
A novel Ncr1-Cre mouse reveals the essential role of STAT5 for NK cell survival and development
Eva Eckelhart
Art der Arbeit
Dissertation
Universität
Universität Wien
Fakultät
Fakultät für Lebenswissenschaften
Betreuer*in
Veronika Sexl
DOI
10.25365/thesis.11640
URN
urn:nbn:at:at-ubw:1-29699.93380.451861-4
Link zu u:search
(Print-Exemplar eventuell in Bibliothek verfügbar)
Abstracts
Abstract
(Deutsch)
Nicht angegeben
Abstract
(Englisch)
We have generated a transgenic mouse line that expresses the Cre recombinase under the control of the NcrI (p46) promoter. Cre mediated recombination was tightly restricted to natural killer (NK) cells as revealed by crossing NcrIiCre mice to the EGFPLSL reporter strain. NcrIiCre mice were further used to study NK cell specific functions of Stat5 by generating Stat5f/f NcrIiCre animals. Stat5f/f NcrIiCre mice were largely devoid of NK cells in peripheral lymphoid organs. In the bone marrow, NK cell maturation was abrogated at the NK cell precursor (NKP) stage. Moreover, we found that in vitro deletion of Stat5 in IL-2 expanded NK cells was incompatible with NK cell viability. In vivo assays confirmed the complete abrogation of NK cell mediated tumor control against B16F10 melanoma cells. In contrast, T cell mediated tumor surveillance against MC38 adenocarcinoma cells was undisturbed. In summary, our study shows a cell intrinsic role for STAT5 for NK cell development and reveals that NcrIiCre mice are a powerful novel tool to study NK cell development, biology and function.
Schlagwörter
Schlagwörter
(Englisch)
NK cells NK cell development NK cell survival NK cell specific Cre Mouse
Schlagwörter
(Deutsch)
Natürliche Killer Zellen NK Zellen NK Zell spezifische Cre Maus Cre Maus Linie
Autor*innen
Eva Eckelhart
Haupttitel (Englisch)
A novel Ncr1-Cre mouse reveals the essential role of STAT5 for NK cell survival and development
Publikationsjahr
2010
Umfangsangabe
VI, 138 S. : Ill., graph. Darst.
Sprache
Englisch
Beurteiler*innen
Wolfgang Doppler ,
Andreas Diefenbach
Klassifikation
42 Biologie > 42.13 Molekularbiologie
AC Nummer
AC08445169
Utheses ID
10504
Studienkennzahl
UA | 091 | 490 | |